Master thesis – Impact of microbial culture conditions on antimicrobial tolerance

Division: R&D Wound Care

The goal of this thesis is to investigate how different ways of pre-culturing microbes (both gram+ and gram- bacteria and yeast) affect subsequent antimicrobial tolerance.

The MSc thesis work can include studies on:

• Microbial viability testing
• Minimal inhibitory and bactericidal concentration testing of different antimicrobial compounds
• Cell counting
• Cell metabolism
• Imaging-based viability analysis
• Microbial aggregation
• Light microscopy
• Scanning electron microscopy
• Dynamic light scattering
• Differential gene expression
• qPCR

The successful candidate should have an interest in microbiology and lab-based work. A curious mindset with an interest in combining different analytical tools is essential. Experience from courses, lab experiments etc in micro- or cell biology is considered a merit. The candidate will carry out the thesis within the group “Pre-clinical Biology and Testing” in well-equipped laboratories.

Supervisor from Mölnlycke is Erik Gerner, questions regarding the thesis work can be email to Erik: Erik.Gerner@molnlycke.com